Close Menu
  • News
  • Medical
  • Technology
  • Nanomaterials
  • Research
  • Blog
    • Nasiol.com
  • Contact
    • Tech7685@gmail.com
What's Hot

A new molecular model of bilayer graphene with higher semiconducting properties

May 31, 2025

5 Nanomaterial Innovations That Didn’t Deliver (Yet)

May 30, 2025

Scientists identify new 2D copper boride material with unique atomic structure

May 30, 2025
Facebook X (Twitter) Instagram
Nanotech – Nanomaterials | Medical | Research | News Stories Updated Daily Nanotech – Nanomaterials | Medical | Research | News Stories Updated Daily
  • News
  • Medical
  • Technology
  • Nanomaterials
  • Research
  • Blog
    • Nasiol.com
  • Contact
    • Tech7685@gmail.com
Facebook X (Twitter) Instagram
Nanotech – Nanomaterials | Medical | Research | News Stories Updated Daily Nanotech – Nanomaterials | Medical | Research | News Stories Updated Daily
Home»News»NMR-guided optimization of lipid nanoparticles for enhanced siRNA delivery
News

NMR-guided optimization of lipid nanoparticles for enhanced siRNA delivery

October 9, 2024No Comments4 Mins Read
Facebook Twitter Pinterest Telegram LinkedIn Tumblr WhatsApp Email
NMR-guided optimization of lipid nanoparticles for enhanced siRNA delivery
Share
Facebook Twitter LinkedIn Pinterest Telegram Email
Diagram of the image of being able to distinguish differences in the state of nucleic acids inside LNPs by evaluating their physical properties using NMR. Credit: Dr. Keisuke Ueda from Chiba University, Japan

Small interfering RNA (siRNA) molecules hold immense potential for treating diseases by silencing specific genes. Encapsulated in lipid nanoparticles (LNPs), siRNA can be delivered efficiently to target cells.

However, the effectiveness of these therapies hinges on the internal structure of the LNPs, which can significantly impact their ability to deliver siRNA. Traditional methods often fall short in providing the detailed molecular insights needed to fine-tune LNP design for optimal therapeutic efficacy.

A study published in the Journal of Controlled Release on August 2, 2024, led by Assistant Professor Keisuke Ueda from the Graduate School of Pharmaceutical Sciences, Chiba University, introduces a novel approach to improving siRNA-loaded LNPs.

By employing NMR-based molecular-level characterization, the research investigates how different siRNA mixing methods affect the uniformity and molecular state of siRNA within LNPs.

The research was coauthored by Dr. Hidetaka Akita from the Graduate School of Pharmaceutical Sciences, Tohoku University; Dr. Kenjirou Higashi from the Graduate School of Pharmaceutical Sciences, Chiba University; and Dr. Kunikazu Moribe (last author) from the Graduate School of Pharmaceutical Sciences, Chiba University.

“NMR allowed us to peer inside these nanoparticles at a molecular level, revealing the intricate details of how siRNA is distributed within the LNP core. This level of insight is crucial for understanding and optimizing LNP formulations,” said Dr. Ueda.

The team compared three preparation methods for siRNA-loaded LNPs to understand their impact on molecular structure and gene-silencing efficiency. The methods included pre-mixing, where siRNA and lipids were combined using a microfluidic mixer; post-mixing (A), where siRNA was mixed with empty LNPs in an acidic condition with ethanol; and post-mixing (B), where siRNA was mixed with empty LNPs in an acidic condition without ethanol.

See also  Nanoparticle-based delivery system could offer treatment for diabetics with rare insulin allergy

While all three methods produced LNPs with a consistent size of about 50nm and maintained a constant ratio of siRNA to lipid content, the distribution of siRNA within the LNPs varied significantly. The pre-mixing method, where siRNA and lipids are mixed simultaneously, resulted in a more uniform distribution of siRNA within the LNPs.

In contrast, the post-mixing method, where siRNA is added to pre-formed LNPs, led to a heterogeneous distribution with regions of high and low siRNA concentration.

“This heterogeneity can significantly impact the silencing effect of the siRNA. LNPs with a more uniform siRNA distribution are more likely to deliver their therapeutic payload to target cells effectively. This highlights the critical need to optimize preparation conditions for improving therapeutic outcomes,” explains Dr. Ueda.

The findings indicate that pre-mixed LNPs exhibit superior gene-silencing effects. In these LNPs, ionizable lipids were more tightly associated with siRNA, forming a stacked bilayer structure that enhanced gene silencing.

In contrast, post-mixed LNPs displayed a more heterogeneous structure, likely impeding their ability to fuse with cell membranes and reducing their therapeutic effectiveness.

“This research could improve people’s lives by enhancing gene therapies and RNA-based medicines. By optimizing how siRNA is delivered using lipid nanoparticles (LNPs), treatments for diseases like cancer, genetic disorders, and viral infections could become more effective. Additionally, it could improve the efficiency and safety of RNA vaccines, like those used for COVID-19, by making them more stable and reducing side effects. Overall, this study has the potential to lead to more effective and safer treatments for patients,” adds Dr. Ueda.

See also  Nanoparticles turbocharge turmeric's curcumin for enhanced health benefits

Looking ahead, these advancements could contribute to the development of more personalized medicine, with treatments tailored to individual patients. Enhanced drug delivery systems could also reduce costs and increase access to innovative therapies, benefiting a wider population.

Provided by
Chiba University



Source link

delivery Enhanced Lipid nanoparticles NMRguided Optimization siRNA
Share. Facebook Twitter Pinterest LinkedIn Tumblr Email

Related Posts

A new molecular model of bilayer graphene with higher semiconducting properties

May 31, 2025

5 Nanomaterial Innovations That Didn’t Deliver (Yet)

May 30, 2025

Scientists identify new 2D copper boride material with unique atomic structure

May 30, 2025

Nanoscale biosensor lets scientists monitor molecules in real time

May 30, 2025

How should we govern nanotechnology?

May 29, 2025

The Future of Needle-Free Immunization

May 28, 2025

Comments are closed.

Top Articles
Medical

Huntington’s disease symptoms reduced by new peptide-polymer therapy

News

XY-Theta Multi-Axis Positioning Stages

News

Carbon-based sensors are poised to facilitate a seamless human-machine interface

Editors Picks

A new molecular model of bilayer graphene with higher semiconducting properties

May 31, 2025

5 Nanomaterial Innovations That Didn’t Deliver (Yet)

May 30, 2025

Scientists identify new 2D copper boride material with unique atomic structure

May 30, 2025

New contact lenses allow wearers to see in the near-infrared

May 30, 2025
About Us
About Us

Your go-to source for the latest nanotechnology breakthroughs. Explore innovations, applications, and implications shaping the future at the molecular level. Stay informed, embrace the nano-revolution.

We're accepting new partnerships right now.

Facebook X (Twitter) Instagram Pinterest
Our Picks

How Does Nanoparticle Tracking Analysis Work?

March 9, 2024

Thinner antiferroelectrics become ferroelectric

August 9, 2023

Drug-loaded hydrogel microelectrode arrays significantly boost brain-computer interface performance, study finds

November 21, 2024

Subscribe to Updates

Get the latest creative Nano Tech news from Elnano.com

© 2025 Elnano.com - All rights reserved.
  • Contact
  • Privacy Policy
  • Terms & Conditions

Type above and press Enter to search. Press Esc to cancel.

Cleantalk Pixel